ABSTRACT Mucins are high molecular mass glycoproteins with oligosaccha-rides O-bonded to the protein core. β-elimination is the most popular method used for releasing of O-glycans. However to such released glycoforms it is difficult to introduce a label to amplify a signal for oligosaccharide detection. In our study we used a combination of the β-elimination and hydrazinolysis methods. Released glycoforms were labeled with para-amino benzooic acid ethyl ester (ABBE) and fractionated on HPLC column. This combined procedure seems to be a good tool for O-glycans analysis
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
Protein O-glycosylation is one of the most diverse post-translational modifications. A critical step...
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
Changes in both N- and O-glycosylation have been associated with many states of health and disease...
The study of protein O-glycosylation is receiving increasing attention in biological, medical, and b...
The study of protein O-glycosylation is receiving increasing attention in biological, medical, and b...
ABSTRACT: The use of hydrazine to release unreduced N- and O-linked oligosaccharides from glycoprote...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional,...
Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional,...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides rele...
A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides rele...
The O-glycosylation of Ser and Thr by N-acetylgalactosamine-linked (mucin-type) oligosaccharides is ...
Important progress has been made recently in the development of analytical technology for O-linked o...
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
Protein O-glycosylation is one of the most diverse post-translational modifications. A critical step...
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
Changes in both N- and O-glycosylation have been associated with many states of health and disease...
The study of protein O-glycosylation is receiving increasing attention in biological, medical, and b...
The study of protein O-glycosylation is receiving increasing attention in biological, medical, and b...
ABSTRACT: The use of hydrazine to release unreduced N- and O-linked oligosaccharides from glycoprote...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional,...
Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional,...
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysa...
A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides rele...
A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides rele...
The O-glycosylation of Ser and Thr by N-acetylgalactosamine-linked (mucin-type) oligosaccharides is ...
Important progress has been made recently in the development of analytical technology for O-linked o...
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
Protein O-glycosylation is one of the most diverse post-translational modifications. A critical step...
Glycosylation improves the solubility and stability of proteins, contributes to the structural integ...
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