Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and organisms represent technical hurdles for the application of HR-mediated knock-in technology. Here, we introduce an alternative strategy for gene knock-in using transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated 9 (Cas9) mediated by microhomology-mediated end-joining, termed the PITCh (Precise Integration into Target Chromosome) system. TALEN-mediated PITCh, termed TAL-PITCh, enables efficient integration ...
Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindrom...
Summary: Intra-embryo genome editing by CRISPR/Cas9 enables easy generation of gene-modified animals...
The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of...
Genome editing technology heralds a new era for animal cell engineering. Programmable site-specific ...
Gene knock-in techniques have rapidly evolved in recent years, along with the development and matura...
Abstract: Gene knock-in techniques have rapidly evolved in recent years, along with the development ...
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) techno...
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated...
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. Ho...
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) s...
CRISPR/Cas9 genome editing is at the forefront of becoming a vital tool for the study of genes and t...
Modifying the genomes of many organisms is becoming as easy as manipulating DNA in test tubes, which...
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and ...
Current approaches for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Ass...
Biopharmaceutical proteins are usually produced by culturing recombinant Chinese hamster ovary (CHO)...
Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindrom...
Summary: Intra-embryo genome editing by CRISPR/Cas9 enables easy generation of gene-modified animals...
The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of...
Genome editing technology heralds a new era for animal cell engineering. Programmable site-specific ...
Gene knock-in techniques have rapidly evolved in recent years, along with the development and matura...
Abstract: Gene knock-in techniques have rapidly evolved in recent years, along with the development ...
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) techno...
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated...
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. Ho...
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) s...
CRISPR/Cas9 genome editing is at the forefront of becoming a vital tool for the study of genes and t...
Modifying the genomes of many organisms is becoming as easy as manipulating DNA in test tubes, which...
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and ...
Current approaches for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Ass...
Biopharmaceutical proteins are usually produced by culturing recombinant Chinese hamster ovary (CHO)...
Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindrom...
Summary: Intra-embryo genome editing by CRISPR/Cas9 enables easy generation of gene-modified animals...
The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of...