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Invasive aspergillosis (IA) is an important cause of morbidity and mortality in immunocompromised individuals. Patients at the highest risk are those with prolonged periods of neutropenia due to chemotherapy for acute leukemia or hematopoietic stem cell transplantation (HSCT) for hematological malignancies or solid tumors [1,2]. Conventional diagnostic tests such as blood cultures are not useful or practical for the diagnosis of Aspergillus spp. fungemia [3]. Further, non-culture–based techniques used in the past lack the sensitivity and specificity needed for the testing of immunocompromised patients [4-7]. Additionally, established IA is difficult to treat and has a death rate of 80–90%, which has driven researchers to develop more reliable and effective methods for its timely diagnosis [1,2,8,9]. Polymerase chain reaction (PCR) assays (conventional, nested, and real-time) have recently bee