Supplemental data

CCR2 on CD141CD161 monocytes is a biomarker of HIV-associated neurocognitive disorders Objective: To evaluate C-C chemokine receptor type 2 (CCR2) on monocyte subsets as a prognos-tic peripheral blood biomarker of HIV-associated neurocognitive disorders (HAND). Methods:Wecharacterizedmonocyte populations in HIV-infected individualswith andwithout HAND from 2 cohorts and assessed their transmigration across an in vitro model of the human blood-brain barrier (BBB). We examined CCR2 expression among the monocyte populations as a prognostic/ predictive biomarker of HAND and its functional consequences in facilitating monocyte diapedesis. Results: We determined that CCR2 was significantly increased on CD141CD161 monocytes in individuals with HAND compared to infected people with normal cognition. CCR2 remained ele-vated irrespective of the severity of cognitive impairment, combined antiretroviral therapy status, viral load, and current or nadir CD4 T-cell count. There was no association between CCR2 on other monocyte populations and HAND. There was a functional consequence to the increase i