RESEARCH Open Access Effect of apolipoprotein A1 genetic

polymorphisms on lipid profiles and the risk of coronary artery disease BiHong Liao1†, KeQi Cheng1†, ShaoHong Dong1, HuaDong Liu1 and ZhengLei Xu2* Background: The disorder of lipid metabolism and genetic predisposition are major risk factors for coronary artery disease (CAD). Variants in the apolipoprotein A1 (APOA1) gene play an important role in the regulation of lipids. The objective of the present study was to investigate the effect of two polymorphisms (-75 G/A and +83 C/T) of APOA1 on lipid profiles and the risk of CAD. Methods: A total number of 300 subjects with CAD and 300 age and sex matched healthy controls were enrolled for the study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing