The Complete Mitochondrial Genome of Brachmia macroscopa (Lepidoptera: Gelechiidae) and Its Related Phylogenetic Analysis

The sweet potato leaf folder, Brachmia macroscopa, is an important pest in China. The complete mitogenome, which consists of 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and an AþT-rich region, was sequenced and found to be 15,394bp in length (GeneBank no. KT354968). The gene order and orientation of the B. macroscopa mitogenome were similar to those of other sequenced lepidopteran spe-cies. All of the PCGs started with ATN as the canonical start codon except for cox1, which started with CGA. In regard to stop codons, most PCGs stopped at TAA except for cox2, which stopped at TA, and nad4, which stopped at a single T. Thirteen PCGs of the available species (33 taxa) were used to demonstrate phylogenetic relationships. The ditrysian cluster was supported as a monophyletic clade at high levels by using maximum likelihood and Bayesian methods. The apoditrysian group, covering the Gelechioidea, formed a monophyletic clade with a bootstrap value of 88 % and a posterior probability of 1.00. The superfamily Gelechioidea was sup-ported as a monophyletic lineage by a posterior probability of 1.00. Key words: Brachmia macroscopa; Gelechioidea; mitogenome; phylogeny As a well-known leaf-eating pest, Brachmia macroscopa Meyrick, belonging to the family Gelechiidae in the superfamily Gelechioidea, occurs in many districts of China, including Shanghai, Zhejiang, Hunan, and Hainan (Wen and Wang 2010), and damages crops by feeding on large amounts of mesophyll (Wang and Tan 2011). This pest is widely distributed outside o