Background: Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analyzing early gene function in Xenopus. However, functional analysis of genes involved in neuronal differentiation and axon pathfinding by this method is often hampered by earlier function of these genes during development. Therefore, fine spatio-temporal control of over-expression or knock-down approaches is required to specifically address the role of a given gene in these processes. Results: We describe here an electroporation procedure that can be used with high efficiency and low toxicity for targeting DNA and antisense morpholino oligonucleotides (MOs) into spatially restricted regions of the Xenopus CNS at a critical time-window of develo...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
Background: Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analy...
Electroporation is an efficient method of delivering DNA and other charged macromolecules into tissu...
In vivo electroporation is a powerful method for delivering DNA expression plasmids, RNAi reagents, ...
Xenopus laevis is an ideal model system for investigating the mechanisms of pattern formation. The a...
Electroporation is becoming more popular as a technique for transfecting neurons within intact tissu...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
In order to understand the function of genes expressed in specific region of the developing brain, i...
For complete information on the use and execution of this protocol, please refer to Cárdenas et al. ...
Summary: In vivo electroporation has become a key technique to study genetic mechanisms of brain dev...
journal articleThe central vocal pathway of the African clawed frog, Xenopus laevis, is a powerful v...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
Background: Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analy...
Electroporation is an efficient method of delivering DNA and other charged macromolecules into tissu...
In vivo electroporation is a powerful method for delivering DNA expression plasmids, RNAi reagents, ...
Xenopus laevis is an ideal model system for investigating the mechanisms of pattern formation. The a...
Electroporation is becoming more popular as a technique for transfecting neurons within intact tissu...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
In order to understand the function of genes expressed in specific region of the developing brain, i...
For complete information on the use and execution of this protocol, please refer to Cárdenas et al. ...
Summary: In vivo electroporation has become a key technique to study genetic mechanisms of brain dev...
journal articleThe central vocal pathway of the African clawed frog, Xenopus laevis, is a powerful v...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...
<p><b>Copyright information:</b></p><p>Taken from "Electroporation of cDNA/Morpholinos to targeted a...