NANO EXPRESS Preparation and optimizat metalloproteinase-1-loade

Full list of author information is available at the end of the articleKeywords: Matrix metalloproteinase-1, PLGA-PCL, Nanoparticles, Rotatable central composite design, Response surface methodology Background Matrix metalloproteinases (MMPs) are a family of enzymes capable of degrading extracellular matrix proteins [1]. This ability is a requirement for cell migration and tissue remodeling, both of which play essential roles in embryonic development, reproduction, tissue remodeling as well as many other physiological and pathological pro-cesses. Matrix metalloproteinase-1 (MMP-1), known as interstitial collagenase, plays a pivotal role in degrading collagen and resolving scar to enhance muscle healing [2-4]. Several investigations have shown that direct injection of proMMP-1 into fibrotic skeletal muscle resulted in the reduction of collagen content without adversely affecting the uninjured muscle. More recently, a poly(ethylene gly