RESEARCH ARTICLE A high density GBS map o rze

Full list of author information is available at the end of the articleBackground Various marker systems, ranging from low-density re-striction fragment length polymorphisms (RFLPs) to high-density single nucleotide polymorphisms (SNPs), have been developed and utilized successfully in wheat for genetic diversity analysis, complex trait dissection, and marker-assisted breeding [1-11]. Anchoring mo-lecular markers on chromosomes and constructing a genetic linkage map are pre-requisites for their utilization in breeding [7,8,10,12-14]. Advances in next-generation technologies have driven the costs of DNA sequencing down to the point that genotyping based on sequence data is now feasible for high diversity, large genome species. Genotyping methods usually involve restriction enzyme digestion of target genomes to reduce the complexity at a reasonable cost [7,15-20]. Davey et al. [17] grouped approaches that apply genome complexity reduction methods into the following classes: reduced-representation sequencing, restriction-site-associated DNA sequencing (RAD-seq), low coverag