Scientific Report The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription

Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. A budding yeast strain with an eco1 mutation that genocopies Roberts syndrome has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. We show that deleting FOB1—a gene that encodes a replication fork-blocking protein specific for the rDNA region— rescues rRNA production and partially rescues transcription genome-wide. Further studies show that deletion of FOB1 corrects the genome-wide replication defects, nucleolar structure, and rDNA segregation that occur in the eco1 mutant. Our study high-lights that the presence of cohesin at the rDNA locus has a central role in controlling global DNA replication and gene expression