Background: A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. Results: Four different aqueous quenching solutions, all containing 60 % of methanol, were compared for their efficiency. Only the solutions containing either 70 mM HEPES or 0.85 % (w/v) ammonium carbonate (pH 5.5) caused less than 10 % cell leakage and the energy charge of the quenched cells was high, indicating rapid inactivation of the metabolism. The efficiency of extraction of intracellular metabolites from cell cultures depends on the extraction methods, and is expected to vary between micro-organisms. For L. plantarum, we have compared fi...
In the present work the effect of quenching on quantification of intracellular metabolites in Coryne...
Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Co...
Capturing a valid snapshot of the metabolome requires rapid quenching of enzyme activities. This is ...
Background A reliable quenching and metabolite extraction method has been developed for Lactobacillu...
Sample preparation is one of the most important steps in metabolome analysis. The challenges of dete...
International audienceIn the present work we investigated the most commonly applied methods used for...
A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous ...
Absolute quantification of free intracellular metabolites is a valuable tool in both pathway discove...
Obtaining meaningful snapshots of the metabolome of microorganisms requires rapid sampling and immed...
Metabolomics aims to quantify all metabolites within an organism, thereby providing valuable insight...
In view of the high citric acid production capacity of Aspergillus niger, it should be well suited a...
International audienceBackground: The gram-positive bacterium Bacillus methanolicus MGA3 is a promis...
In this study, exemplified by Escherichia coli, a sample preparation method was developed and evalua...
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become import...
Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of th...
In the present work the effect of quenching on quantification of intracellular metabolites in Coryne...
Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Co...
Capturing a valid snapshot of the metabolome requires rapid quenching of enzyme activities. This is ...
Background A reliable quenching and metabolite extraction method has been developed for Lactobacillu...
Sample preparation is one of the most important steps in metabolome analysis. The challenges of dete...
International audienceIn the present work we investigated the most commonly applied methods used for...
A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous ...
Absolute quantification of free intracellular metabolites is a valuable tool in both pathway discove...
Obtaining meaningful snapshots of the metabolome of microorganisms requires rapid sampling and immed...
Metabolomics aims to quantify all metabolites within an organism, thereby providing valuable insight...
In view of the high citric acid production capacity of Aspergillus niger, it should be well suited a...
International audienceBackground: The gram-positive bacterium Bacillus methanolicus MGA3 is a promis...
In this study, exemplified by Escherichia coli, a sample preparation method was developed and evalua...
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become import...
Metabolomics is a comprehensive analysis of metabolites existing in biological systems. As one of th...
In the present work the effect of quenching on quantification of intracellular metabolites in Coryne...
Messenger RNA levels change on a minutes scale due to both degradation and de novo transcription. Co...
Capturing a valid snapshot of the metabolome requires rapid quenching of enzyme activities. This is ...