Summary

With the advent of high-resolution mass spectrometry together with sophisticated data analysis and inter-pretation algorithms, determination of protein synthe-sis and degradation rates (i.e. protein turnover) on a proteome-wide scale by employing stable isotope-labelled amino acids has become feasible. These dynamic data provide a deeper understanding of protein homeostasis and stress response mecha-nisms in microorganisms than well-established ‘steady state ’ proteomics approaches. In this article, we summarize the technological challenges and solu-tions both on the biochemistry/mass spectrometry and bioinformatics level for turnover proteomics with a focus on chromatographic techniques. Although the number of available case studies for Corynebac