Estimation the Frequency of Human Immunodeficiency Virus among Male and Female Patients, Iran 0DVRXG+DMLD3K'1 $OL$PLU]DUJDU3K'20HKGL*KRUHLVKL06F36RKUDE6DP%6F4 'HSDUWPHQWRI0HGLFDO0LFURELRORJ\+HDOWK5HIHUHQFH/DERUDWRU\RI,UDQ0LQLVWU\RI+HDOWKDQG

2EMHFWLYH A reduction in new human immunodeficiency virus(HIV) cases is one of the ten areas prioritized by the United Nations Program on HIV. However, recent official re-ports confirm the HIV rate is increasing and predicted a huge incidence in the near future in Iran, despite the preventative program by Iran’s Health Ministry. In this descriptive study, we evaluate the frequency of HIV positive cases among referral patients to a private clinic laboratory for its diagnosis in addition to specimens from other laboratories. An epidemio-logical analysis is also performed. 0DWHULDOVDQG0HWKRGV In this descriptive study, the total number of patients was 138 cases that referred for the diagnosis of HIV to the private Laboratory. Of these, 93 males DQGIHPDOHV YROXQWDULO\UHTXHVWHGWREHH[DPLQHGIRUVSHFLILFLQFUHDV-es in specific antibody titer, western blot assays and RNA quantitation polymerase chain reaction. We collected two separate tubes of whole blood, one for reverse transcriptase-polymerase chain reaction analysis and the second one for the remaining two tests. Those patients who were antibody positive by western blot and/or reverse transcriptase-polymer