Pancreatic stellate cells (PSC) are involved in fibrogenesis and oncogenesis by modulating the extracellular matrix. Aim: To evaluate the effect of cellular stress on PSC activation using a model of normal human pancreatic tissue slices culture preserving the microenvironment. Methods: Thin sections (300μm) of normal human pancreas were cultured under hyperoxia (90 % O2) during 72 hours. Viability and morphological analysis were performed at baseline, H24, H48 and H72. Cell differentiation (insulin, trypsin, CA9 and CK7), hypoxia (HIF1-α), apoptosis (caspase-3), proliferation (Ki67), TGF-β expression and PSC activation (smooth muscle actin (SMA), nestin) were assessed using immunostaining, longitudinally. Control experiments were performed ...
Pancreatic stellate cells (PaSCs) are non-endocrine, mesenchymal-like cells that reside within the p...
Tissue engineering is evolving to mimic intricate ecosystems of tumour microenvironments (TME) to mo...
Stromal fibrosis and tissue necrosis are major histological sequelae of hypoxia. The hypoxia-to-fibr...
Pancreatic stellate cells (PSC) are involved in fibrogenesis and oncogenesis by modulating the extra...
To evaluate the effect of cellular stress on PSC activation using a model of normal human pancreatic...
Background: Pancreatic stellate cells (PSCs) play a critical role in pancreatic fibrosis. To date, h...
BACKGROUND AND AIMS: Although both cancer and stellate cells (PSCs) secrete proangiogenic factors, p...
Pancreas fibrosis is the result of a dynamic cascade of mechanisms beginning with acinar cell (AC) i...
While the morphology and function of cells of the exocrine and endocrine pancreas have been studied ...
<p>(<b>A</b>) For hypoxia induction <i>in vitro</i>, BxPc-3 and MIA-PaCa2 cells were incubated in an...
AbstractBackground and Aims Although both cancer and stellate cells (PSCs) secrete proangiogenic fac...
Pancreatic stellate cells (PSC) play a central role in fibrogenesis associated with acute and chroni...
The mechanisms of pancreatic fibrosis are poorly understood. In the liver, stellate cells play an im...
Abstract Until now, no specific therapies are available to inhibit pancreatic fibrosis, a constant p...
Purpose of review Ever since the first descriptions of methods to isolate pancreatic stellate cells ...
Pancreatic stellate cells (PaSCs) are non-endocrine, mesenchymal-like cells that reside within the p...
Tissue engineering is evolving to mimic intricate ecosystems of tumour microenvironments (TME) to mo...
Stromal fibrosis and tissue necrosis are major histological sequelae of hypoxia. The hypoxia-to-fibr...
Pancreatic stellate cells (PSC) are involved in fibrogenesis and oncogenesis by modulating the extra...
To evaluate the effect of cellular stress on PSC activation using a model of normal human pancreatic...
Background: Pancreatic stellate cells (PSCs) play a critical role in pancreatic fibrosis. To date, h...
BACKGROUND AND AIMS: Although both cancer and stellate cells (PSCs) secrete proangiogenic factors, p...
Pancreas fibrosis is the result of a dynamic cascade of mechanisms beginning with acinar cell (AC) i...
While the morphology and function of cells of the exocrine and endocrine pancreas have been studied ...
<p>(<b>A</b>) For hypoxia induction <i>in vitro</i>, BxPc-3 and MIA-PaCa2 cells were incubated in an...
AbstractBackground and Aims Although both cancer and stellate cells (PSCs) secrete proangiogenic fac...
Pancreatic stellate cells (PSC) play a central role in fibrogenesis associated with acute and chroni...
The mechanisms of pancreatic fibrosis are poorly understood. In the liver, stellate cells play an im...
Abstract Until now, no specific therapies are available to inhibit pancreatic fibrosis, a constant p...
Purpose of review Ever since the first descriptions of methods to isolate pancreatic stellate cells ...
Pancreatic stellate cells (PaSCs) are non-endocrine, mesenchymal-like cells that reside within the p...
Tissue engineering is evolving to mimic intricate ecosystems of tumour microenvironments (TME) to mo...
Stromal fibrosis and tissue necrosis are major histological sequelae of hypoxia. The hypoxia-to-fibr...