FULL PAPER Physiology Molecular Genetic Diversity of the Gyeongju Donggyeong Dog in Korea

ABSTRACT. The present study was conducted to analyze the genetic characteristics of the Donggyeong dog and establish parentage conserva-tion systems for it by using 10 microsatellite markers recommended by the International Society for Animal Genetics (ISAG). A total of 369 dogs from 12 dog breeds including the Donggyeong dog were genotyped using 10 microsatellite loci. The number of alleles per locus varied from 5 to 10 with a mean value of 7.6 in the Donggyeong dog. The observed heterozygosity and expected heterozygosity ranged from 0.4706 to 0.9020 (mean 0.7657) and from 0.4303 to 0.8394 (mean 0.7266), respectively. The total exclusion probability of 10 microsatellite loci was 0.99955. Of the 10 microsatellite markers, the AHT121, AHTh260 and CXX279 markers had relatively high PIC values (≥0.7). This study found that there were specific alleles, 116 allele at AHT121 in the Donggyeong dog when compared with other dog breeds. Also, the results showed two (Korean native dogs and the foreign dog breeds) distinct clusters. The closest distance (0.1184) was observed between the Donggyeong dog and Jindo dog, and the longest distance (0.3435) was observed between the Donggyeong dog and Bulgae. The Korean native dog breeds have comparatively near genetic distances between each other. KEY WORDS: Donggyeong dog, genetic distance, microsatellite marker doi: 10.1292/jvms.14-0189; J. Vet. Med. Sci. 76(10): 1359–1365, 2014 Dogs had been with us longer than any other domestic animals. Men and dogs share the closest and most intimate relationship. About 400 breeds are distributed worldwide