Research Article The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during In�ammation Does Not Depend on Serum Amyloid A

License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Studies suggest that in�ammation impairs reverse cholesterol transport (RCT). We investigated whether serum amyloid A (SAA) contributes to this impairment using an established macrophage-to-feces RCT model. Wild-type (WT) mice and mice de�cient in SAA1.1 and SAA2.1 (SAAKO) were injected intraperitoneally with 3H-cholesterol-labeled J774 macrophages 4 hr aer administration of LPS or buffered saline. 3H-cholesterol in plasma 4 hr aer macrophage injection was signi�cantly reduced in both WT and SAAKO mice injected with LPS, but this was not associated with a reduced capacity of serum from LPS-injected mice to promote macrophage cholesterol efflux in vitro. Hepatic accumulation of 3H-cholesterol was unaltered in either WT or SAAKOmice by LPS treatment. Radioactivity present in bile and feces of LPS-injected WT mice 24 hr aer macrophage injection was reduced by 36 %