Funding Information

This study was to investigate the absorption and antioxidant effect of a mango-steen-based functional beverage in humans. The beverage contained mango-steen, aloe vera, green tea, and multivitamins. A randomized, double-blind, placebo-controlled clinical trial was conducted with generally healthy male and female subjects between 18 and 60 years of age. Ten men and 10 women partic-ipated in this study. Participants were randomly divided into two groups, treat-ment and placebo group. Participants received either a daily single dose (245 mL) of the beverage or a placebo. Blood samples were collected from each participant at time points 0, 1, 2, 4, and 6 h. The plasma samples were analyzed by LC/MS for a-mangostin and vitamins B2 and B5. Results indicated that the three analytes were bioavailable, with observed Cmax at around 1 h. The antiox-idant capacity measured with the oxygen radical absorbance capacity (ORAC) assay was increased with a maximum effect of 60 % after 1 h, and the elevated antioxidant level lasted at least 6 h. This study demonstrated the bioavailability of a-mangostin and B vitamins from a xanthone-rich beverage and the mecha-nisms of the increase in plasma antioxidant may be direct effects from antioxi-dants, enhancement of endogenous antioxidant activity through activation of Nrf2 pathway, and synergism of the antioxidants