Research Article Mutation Analysis of JAK2V617F, FLT3-ITD, NPM1, and DNMT3A in Chinese Patients with

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Since the discovery of JAK2V617F tyrosine kinase-activatingmutation, several genes have been foundmutated inmyeloproliferative neoplasms (MPNs). FLT3-ITD, NPM1, and DNMT3A mutations frequently occurred in AML patients and have been found conferred with myeloproliferative neoplasms in mouse model. Therefore, we sought to search for mutations in JAK2V617F, FLT3-ITD, NPM1, and DNMT3A in 129 cases including 120 classic MPN cases and 9 MDS/MPN cases. JAK2V617F mutation was found in 60 % of the 120 classic MPNs. However, none of the patients displayed FLT3-ITD and NPM1 mutations; only 2 patients harboredDNMT3AR882mutation. Further studies includingwhole-genome sequencewill be conducted to investigate the possible involvement of these genes in MPN. 1