Research Article Specificity of Sensory and Motor Neurons Associated with BL40 and GB30 in the Rat: A Dual Fluorescent Labeling Study

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The purpose of this study is to investigate the specific innervations on “Weizhong ” (BL40) and “Huantiao ” (GB30) by using a dual neural tracing technique. After Alexa Fluor 488 and 594 conjugates of cholera toxin subunit B (AF488/594-CTB) were, respectively, injected into BL40 and GB30 in the same rat, the labeled sensory and motor neurons were examined in the rat’s dorsal root ganglia (DRGs) and spinal cord at thoracic (T) and lumbar (L) segments with a laser scanning confocal microscope. In the cases of BL40 injection, AF488-CTB labeled sensory andmotor neurons were located in L 2−6 DRGs and on the mediolateral part of spinal ventral horn from L 3 to L 5 segments, respectively. By contrast, in the cases of GB30 injection, AF594-CTB labeled sensory and motor neurons were distributed in