RESEARCH ARTICLE Molecular Cloning and Characterisation of

Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophos-phate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSsmain-tained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSsmight play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the ground-work for the future study of the synthetic biology of natural terpene products