Genome-Wide Analysis of miRNA Signature in the APPswe/PS1DE9 Mouse Model of Alzheimer’s Disease

Alzheimer’s disease (AD) is the most common cause of dementia. One of the pathological hallmarks of AD is amyloid b (Ab) deposition. MicroRNAs (miRNAs) are small non-coding RNAs whose expression levels change significantly during neuronal pathogenesis and may be used as diagnostic markers. Some miRNAs are important in AD development by targeting genes responsible for Abmetabolism. However, a systematic assessment of the miRNA expression profile induced by Ab-mediated neuronal pathogenesis is still lacking. In the present study, we examined miRNA expression profile by using the APPswe/ PS1DE9 mouse model of AD. Two sibling pairs of mice were examined, showing 30 and 24 miRNAs with significantly altered expression levels from each paired control, respectively. Nine known miRNAs were common in both groups. Prediction of putative target genes and functional annotation implied that these altered miRNAs affect many target genes mainly involved in PI3K/Akt signaling pathway. This study provides a general profile of miRNAs regulated by Ab-associated signa