NOTE Internal Medicine Evaluation of Neutrophil Function during Hemodialysis Treatment in Healthy Dogs under Anesthesia with Sevoflurane

ABSTRACT. This study evaluated the number and function of neutrophils during 3 hr of hemodialysis in healthy dogs under anesthesia. Isolated neutrophils were used to assess neutrophil adhesion, phagocytosis and the oxidative burst. At 0.5 and 3 hr after the start of hemodialysis treatment, there was a decrease in neutrophil number. The phagocytic ability of neutrophils was decreased 3 hr after the start of hemodialysis. In conclusion, this study demonstrated that hemodialysis reduces the number and phagocytic ability of neutrophils during treatment. However, these changes recover within 24 hr of hemodialysis. KEY WORDS: canine, hemodialysis, immune function, neutrophil function doi: 10.1292/jvms.13-0541; J. Vet. Med. Sci. 76(11): 1539–1543, 2014 Intermittent hemodialysis (HD) is a useful treatment that improves the outcome in dogs and cats with kidney injuries that do not respond to medical management. Indications for intermittent HD include drug or toxin ingestion, acute or acute-on-chronic kidney injury and chronic kidney disease [4]. During HD, there is an early, transient decrease in the number of polymorphonuclear cells [19]. In dogs, this tran