C 2014 American Chemical Society Triggering of RNA Interference with

NA interference (RNAi) is a natural cellular post-transcriptional gene reg-ulation process that utilizes small double-stranded RNAs to trigger guided gene silencing.1 By introducing synthetic RNA duplexes called small-interfering RNAs (siRNAs),2 we can harness the RNAi machin-ery for therapeutic gene control and the treatment of various diseases.36 RNA nanotechnology offers an advantage of pre-cise control over the composition and stoi-chiometry of the delivered cargo.5,715 RNA molecules can be programmed7,8,1623 to form a wide variety of compact and stable artificial three-dimensional nanostructure