Research Article Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin

Copyright © 2013 Shuqiang Zhao et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Protein fusion technology is one of themost commonly usedmethods to extend the half-life of therapeutic proteins. In this study, in order to prolong the half-life of Granulocyte colony stimulating factor (G-CSF), the domain III of human serum albumin (3DHSA) was genetically fused to the N-terminal of G-CSF. The 3DHSA-G-CSF fusion gene was cloned into pPIC