RESEARCH ARTICLE Ethyl acetate extract of W i s

Full list of author information is available at the end of the articleantioxidant defense mechanism. The brain is especially vulnerable to oxidative stress compared to other organs because it exhibits lower antioxidant enzyme activity and contains an abundance of unsaturated fatty acids, which are the targets of lipid peroxidation. Thereafter oxidative stress contributes to the neurodegenerative disorders, and is one of the earliest pathological changes in Alzheimer’s disease [1]. Apart from quenching free radicals, antioxidants may medicinal herbs catch attention on avoid unwanted neuropathophysiology. Tertiary butyl hydroperoxide (t-BHP), a simple lipophilic alkyl hydroperoxide, has been reported to induce dose-dependent oxidative stress and damage in brain cells [2]. The alkoxyl and alkyl radicals derived from t-BHP are a species that produce cell damage [3,4]. Furthermore, t-BHP-induces a cellular redox imbalance may induce cell apoptosis [5]. Therefore, t-BHP is a useful inducer for investigating the effectiveness of various neuropro-tection products against oxidative stress. In addition, D-galactose at normal concentrations can be metabo