Histological Criteria for Encapsulating Peritoneal Sclerosis – A Standardized Approach

Background: The two most relevant pathologies of long-term peritoneal dialysis (PD) are simple sclerosis and encapsulating peritoneal sclerosis (EPS). The histological differentiation of those two entities is difficult. The Aim of the study was to establish a method to standardize and facilitate the differentiation between simple sclerosis and EPS Methods: We investigated 58 peritoneal biopsies- 31 EPS patients and 27 PD patients. Two blinded investigators analyzed 20 histological characteristics in EPS and PD patients. Results: The following findings were significantly more common in EPS than in patients on PD without EPS: fibroblast like cells (FLC) (p,0.0001), mesothelial denudation (p,0.0001), decreased cellularity (p = 0.008), fibrin deposits (p,0.03), Fe deposits (p = 0.05), podoplanin vascular (p,0.0001), podoplanin avascular (p,0.0001). Using all predictor variables we trained the classification method Random Forest to categorize future cases. Podoplanin vascular and avascular were taken together (p,0.0001), FLC (p,0.0001), mesothelial denudation (p = 0.0005), calcification (p = 0.0026), acellular areas (p = 0.0094), and fibrin deposits (p = 0.0336) showed up as significantly important predictor variables. Estimated misclassification error rate when classifying new cases turned out to be 14%. Conclusion: The introduced statistical method allows discriminating between simple sclerosis and EPS. The misclassificatio