The Effect of Fibroblast Co-culture on In Vitro Maturation of

ABSTRACT: This study was performed to evaluate the effects of fibroblast co-culture on in vitro maturation (IVM) of prepubertal mouse preantral follicles. The intact preantral follicles were obtained from the ovaries of 12 14 day old mice and these were cultured individually in α-minimal essential medium (α-MEM) supplemented with 5 % fetal bovine serum (FBS), 100 mIU/ ㎖ recombinant follicle stimulating hormone (rFSH), 1 % insulin-transferrin-selenium, 100 μg/ml penicillin and 50 ㎖/ ㎖ streptomycin as base medium for 12 days. A total of 200 follicles were cultured in base medium co-cultured with mouse embryonic fibroblast (MEF) (MEF group) (n=100) or only base medium as control group (n=100). Survival rate of follicles on day 12 of culture were significantly higher in the MEF group of 90.0%, compared with 77.0 % of the control group (p=0.021). Follicle diameters on day 6 and 8 of the culture period were significantly larger in the MEF group than those in the control group (p=0.021, p=0.007, respectively). Estradiol levels in culture media on day 4, 6, 8, 10 and 12 of the culture period were significantly higher in the MEF group (p=0.043, p=0.021, p=0.006, p<0.001 and p=0.008, retrospectively). Our data suggest that MEF cell co-culture on IVM of mouse preantral follicle increases survival rate and promotes follicular growth and steroid production. Key words: Co-culture, In vitro maturation, Mouse preantral follicles, Mouse embryonic fibroblas