RESEARCH ARTICLE Complete Genome Sequence of a High Lipid- Producing Strain ofMucor circinelloidesWJ11 and Comparative Genome Analysis with a

The genome of a high lipid-producing fungusMucor circinelloidesWJ11 (36 % w/w lipid, cell dry weight, CDW) was sequenced and compared with that of the low lipid-producing strain, CBS 277.49 (15 % w/w lipid, CDW), which had been sequenced by Joint Genome Institute. TheWJ11 genome assembly size was 35.4 Mb with a G+C content of 39.7%. The general features of WJ11 and CBS 277.49 indicated that they have close similarity at the level of gene order and gene identity. Whole genome alignments with MAUVE revealed the pres-ence of numerous blocks of homologous regions and MUMmer analysis showed that the genomes of these two strains were mostly co-linear. The central carbon and lipid metabo-lism pathways of these two strains were reconstructed and the numbers of genes encoding the enzymes related to lipid accumulation were compared. Many unique genes coding for proteins involved in cell growth, carbohydrate metabolism and lipid metabolism were identi-fied for each strain. In conclusion, our study on the genome sequence of WJ11 and the com-parative genomic analysis between WJ11 and CBS 277.49 elucidated the general features of the genome and the potential mechanism of high lipid accumulation in strain WJ11 at the genomic level. The different numbers of genes and unique genes involved in lipid accumu-lation may play a role in the high oleaginicity of strain WJ11