P.: Temporal Pixel Multiplexing for Simultaneous High-Speed, High-Resolution Imaging. Nature Methods 7

We introduce an imaging modality that, by offsetting pixel exposure times during capture of a single image frame, embeds temporal information within each frame. This allows simultaneous acquisition of full-resolution images at native detector frame-rates, and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. The method is demonstrated using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250Hz using a 10Hz megapixel camera. Conventional cameras capture images at a fixed spatial and temporal resolution. Events that occur faster than the detector integration time (ti) appear blurred (if moving), as an average light intensity (if changing intensity while stationary), or a combination thereof. We propose a temporal pixel multiplexing (TPM) paradigm for imaging that allows simultaneous high-speed and high-resolution image capture on a single detector1. The method is based on subdividing a charge coupled device (CCD) array into m non-overlapping groups of n pixels (Fig. 1, Supplementary Fig. 1). Individual pixels in each group capture their part of the imaged scene sequentially so that at any instant, m spatiall