Research Article In Vitro Ion Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks of Tetrapleura tetraptera and Their Protective Effects against Fenton Mediated Toxicity of Metal

Copyright © 2015 Bruno Moukette Moukette et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH∙), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS∙), hydroxyl radical (HO∙), and nitric oxide (NO∙)), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents.The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC.The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC 50 value with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00CAE/g dried extract); flavonoids (11.36 ± 1.88QE/g dried extract); and flavonols contents (3.95 ± 0.39QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00mg/gDW), quercetin (353.78 ± 00mg/gDW), and rutin (210.5