Add to ORKGHere we describe the development and validation of a highly sensitive assay of antigen-specific IFN-c production using real time quantitative PCR (qPCR) for two reporters- monokine-induced by IFN-c (MIG) and the IFN-c inducible protein-10 (IP10). We developed and validated the assay and applied it to the detection of CMV, HIV and Mycobacterium tuberculosis (MTB) specific responses, in a cohort of HIV co-infected patients. We compared the sensitivity of this assay to that of the ex vivo RD1 (ESAT-6 and CFP-10)-specific IFN-c Elispot assay. We observed a clear quantitative correlation between the two assays (P,0.001). Our assay proved to be a sensitive assay for the detection of MTB-specific T cells, could be performed on whole blood samples ...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
T-cell mediated immunity likely plays an important role in controlling HIV-1 infection and progressi...
BACKGROUND:Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for i...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Background: The ability to measure T-cell responses to antigens is proving critical in the field of ...
Background: The suboptimal sensitivity of Interferon (IFN)-c-based in-vitro assays, especially in im...
Background: The ability to measure T-cell responses to antigens is proving critical in the field of ...
The suboptimal sensitivity of Interferon (IFN)-γ-based in-vitro assays, especially in immunocompromi...
BACKGROUND: The ability to measure T-cell responses to antigens is proving critical in the field of ...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
Interferon (IFN)-γ release assays have limited sensitivity and cannot differentiate between active t...
Background: Antigen specific release of IP-10 is the most promising alternative marker to IFN-c for ...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
T-cell mediated immunity likely plays an important role in controlling HIV-1 infection and progressi...
BACKGROUND:Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for i...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-...
Background: The ability to measure T-cell responses to antigens is proving critical in the field of ...
Background: The suboptimal sensitivity of Interferon (IFN)-c-based in-vitro assays, especially in im...
Background: The ability to measure T-cell responses to antigens is proving critical in the field of ...
The suboptimal sensitivity of Interferon (IFN)-γ-based in-vitro assays, especially in immunocompromi...
BACKGROUND: The ability to measure T-cell responses to antigens is proving critical in the field of ...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
Interferon (IFN)-γ release assays have limited sensitivity and cannot differentiate between active t...
Background: Antigen specific release of IP-10 is the most promising alternative marker to IFN-c for ...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
Monitoring of latent Mycobacterium tuberculosis infection may prevent disease. We tested an ESAT-6 a...
T-cell mediated immunity likely plays an important role in controlling HIV-1 infection and progressi...
BACKGROUND:Antigen specific release of IP-10 is the most promising alternative marker to IFN-γ for i...