pr

Modelling enzymatic cascade networks, however, requires the simulation of multiple reactions. This inevitably increases the com-plexity of the ODE model, which increases the number of free kinetic parameters. It then becomes more difficult to constrain all parameters simultaneously using a limited amount of available y kinetic features modelled based on e kinetics within which additional interactions such as inh and allosteric effects can be included using mass action k [6]. Basic enzyme kinetics is modelled using the following of reactions: Sþ E kaf kdf ES kcr kcf EP kdr kar P þ E ð1Þ where S, E, ES, EP and P denote the substrate, enzyme, enzyme-substrate complex, enzyme-product complex and product, respectively