Bioimage informatics Advance Access publication May 16, 2013 TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization

Motivation: The cell nucleus is a highly organized cellular organelle that contains the genetic material. The study of nuclear architecture has become an important field of cellular biology. Extracting quantita-tive data from 3D fluorescence imaging helps understand the func-tions of different nuclear compartments. However, such approaches are limited by the requirement for processing and analyzing large sets of images. Results: Here, we describe Tools for Analysis of Nuclear Genome Organization (TANGO), an image analysis tool dedicated to the study of nuclear architecture. TANGO is a coherent framework allow-ing biologists to perform the complete analysis process of 3D fluores-cence images by combining two environments: Image