Research Article P2X7 Receptor Activation Induces Reactive Oxygen Species

Copyright © 2013 Rachael Bartlett et al.is is an open access article distributed under theCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. e P2X7 purinergic receptor is a ligand-gated cation channel expressed on leukocytes including microglia. is study aimed to determine if P2X7 activation induces the uptake of organic cations, reactive oxygen species (ROS) formation, and death in the murinemicroglial EOC13 cell line. Using themurinemacrophage J774 cell line as a positive control, RT-PCR, immunoblotting, and immunolabelling established the presence of P2X7 in EOC13 cells. A cyto�uorometric assay demonstrated that the P2X7 agonists adenosine-5�-triphosphate (ATP) and 2�(3�)-O-(4-benzoylbenzoyl) ATP induced ethidium+ or YO-PRO-12+ uptake into both cell lines. ATP induced ethidium+ uptake into EOC13 cells in a concentration-dependent manner, with an EC50 of ∼13