Abstract MicroRNAs are short non-coding RNAs that play an important role in the regulation of gene expression. Hence, microRNAs are considered as potential targets for engineering of Chinese hamster ovary (CHO) cells to improve recombinant protein production. Here, we analyzed and compared the microRNA expression patterns of high, low, and non-producing recombinant CHO cell lines expressing two struc-turally different model proteins in order to identify microRNAs that are involved in heterologous protein synthesis and secre-tion and thus might be promising targets for cell engineering to increase productivity. To generate reproducible and comparable data, the cells were cultivated in a bioreactor under steady-state conditions. Global microR...
Recombinant therapeutic proteins are widely used to treat life-threatening diseases including cancer...
AbstractmiRNAs negatively regulate gene expression at post-transcriptional level, and consequently p...
microRNAs with their ability to regulate complex pathways that control cellular behavior and phenoty...
AbstractAs Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human...
Stiefel F, Fischer S, Sczyrba A, Otte K, Hesse F. miRNA profiling of high, low and non-producing CHO...
Hackl M, Jakobi T, Blom J, et al. Next-generation sequencing of the Chinese hamster ovary microRNA t...
As Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human-like re...
Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production of hu...
The majority of cell engineering for recombinant protein production to date has relied on traditiona...
MicroRNAs (miRs) are a class of non-coding RNA that function to regulate global mammalian gene expre...
AbstractChinese hamster ovary (CHO) cells are the predominant cell factory for the production of rec...
Chinese Hamster Ovary (CHO) cells are the prominent cell line used in biopharmaceutical production. ...
MikroRNAs (miRNAs) sind kurze regulatorische RNA-moleküle, welche durch spezifische Bindung die Gene...
Chinese hamster ovary (CHO) cells are the most commonly used cell line for the production of recombi...
To date the major advances in biopharmaceutical production from Chinese Hamster Ovary (CHO) cells ha...
Recombinant therapeutic proteins are widely used to treat life-threatening diseases including cancer...
AbstractmiRNAs negatively regulate gene expression at post-transcriptional level, and consequently p...
microRNAs with their ability to regulate complex pathways that control cellular behavior and phenoty...
AbstractAs Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human...
Stiefel F, Fischer S, Sczyrba A, Otte K, Hesse F. miRNA profiling of high, low and non-producing CHO...
Hackl M, Jakobi T, Blom J, et al. Next-generation sequencing of the Chinese hamster ovary microRNA t...
As Chinese Hamster Ovary (CHO) cells are the cell line of choice for the production of human-like re...
Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production of hu...
The majority of cell engineering for recombinant protein production to date has relied on traditiona...
MicroRNAs (miRs) are a class of non-coding RNA that function to regulate global mammalian gene expre...
AbstractChinese hamster ovary (CHO) cells are the predominant cell factory for the production of rec...
Chinese Hamster Ovary (CHO) cells are the prominent cell line used in biopharmaceutical production. ...
MikroRNAs (miRNAs) sind kurze regulatorische RNA-moleküle, welche durch spezifische Bindung die Gene...
Chinese hamster ovary (CHO) cells are the most commonly used cell line for the production of recombi...
To date the major advances in biopharmaceutical production from Chinese Hamster Ovary (CHO) cells ha...
Recombinant therapeutic proteins are widely used to treat life-threatening diseases including cancer...
AbstractmiRNAs negatively regulate gene expression at post-transcriptional level, and consequently p...
microRNAs with their ability to regulate complex pathways that control cellular behavior and phenoty...