Abstract To understand how and when developmental traits of the fruit fly Drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. The experimental toolkit available for relevant fly models typically comprises gene expression and loss as well as gain-of-function analyses. Here, we extend the set of available molecular tools to piggyBac-based germ line transformation in two satellite fly models, Megaselia abdita and Chironomus riparius. As proof-of-concept application, we used a Gateway variant of the piggyBac transposon vector pBac{3xP3-eGFPafm} to generate a transgenic line that expresses His2Av-mCherry as fluorescent nuclear reporter ubiquitous...
The sterile insect technique (SIT) is concerned with the mass-rearing and release of sterilized inse...
Germ line transformation is a powerful research tool to probe and manipulate gene function but curre...
BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive pes...
We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, usi...
The piggyBac transposable element was successfully used for stable genetic transformation of the hou...
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauri...
Developing biological systems can be approximately described as complex, three dimensional cellular ...
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauri...
Background: Most of the known genes required for developmental processes have been identified by gen...
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly...
Background: Stalk-eyed flies of the family Diopsidae have proven to be an excellent model organism f...
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly...
Stalk-eyed flies of the family Diopsidae have their eyes laterally displaced on the end of head exte...
The fruit fly Drosophila melanogaster represents a key model organism for analyzing how neuronal cir...
Stable and efficient germ-line transformation was achieved in the South American malaria vector, Ano...
The sterile insect technique (SIT) is concerned with the mass-rearing and release of sterilized inse...
Germ line transformation is a powerful research tool to probe and manipulate gene function but curre...
BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive pes...
We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, usi...
The piggyBac transposable element was successfully used for stable genetic transformation of the hou...
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauri...
Developing biological systems can be approximately described as complex, three dimensional cellular ...
Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauri...
Background: Most of the known genes required for developmental processes have been identified by gen...
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly...
Background: Stalk-eyed flies of the family Diopsidae have proven to be an excellent model organism f...
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly...
Stalk-eyed flies of the family Diopsidae have their eyes laterally displaced on the end of head exte...
The fruit fly Drosophila melanogaster represents a key model organism for analyzing how neuronal cir...
Stable and efficient germ-line transformation was achieved in the South American malaria vector, Ano...
The sterile insect technique (SIT) is concerned with the mass-rearing and release of sterilized inse...
Germ line transformation is a powerful research tool to probe and manipulate gene function but curre...
BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive pes...