The unwinding of nucleic acid secondary structures within cells is crucial to maintain genomic integrity and prevent abortive transcription and translation initiation. DHX36, also known as RHAU or G4R1, is a DEAH-box ATP-dependent helicase highly specific for DNA and RNA G-quadruplexes (G4s). A funda-mental mechanistic understanding of the interaction between helicases and their G4 substrates is impor-tant to elucidate G4 biology and pave the way toward G4-targeted therapies. Here we analyze how the ther-modynamic stability of G4 substrates affects bind-ing and unwinding by DHX36. We modulated the sta-bility of the G4 substrates by varying the sequence and the number of G-tetrads and by using small, G4-stabilizing molecules. We found an inv...
Eukaryotic translation initiation is a highly regulated process in protein synthesis. The principal ...
Helicases, enzymes that unwind DNA or RNA struc-ture, are present in the cell nucleus and in the mit...
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently...
The unwinding of nucleic acid secondary structures within cells is crucial to maintain genomic integ...
Guanine-rich nucleic acid sequences challenge the replication, transcription, and translation machin...
The concept that G-quadruplex (G4) structures can form within DNA or RNA in vitro has been long know...
Four-stranded nucleic acid structures called G-quadruplexes have been associated with important cell...
G-quadruplex (or G4) structures form in guanine-rich DNA sequences and threaten genome stability whe...
DFAD-box helicases are ubiquitous enzymes that catalyze the unwinding of energetically stable duplex...
Translation efficiency can be affected by mRNA stability and secondary structures, including G-quadr...
The identification of G-quadruplex (G4) binding proteins and insights into their mechanism of action...
G-quadruplexes (G4) are RNA and DNA secondary structures formed by the stacking of guanine quartets ...
International audienceG-rich sequences found at multiple sites throughout all genomes may form secon...
Members of the DEAD-box helicase family are involved in all fundamental processes of RNA metabolism,...
Eukaryotic translation initiation is a highly regulated process in protein synthesis. The principal ...
Helicases, enzymes that unwind DNA or RNA struc-ture, are present in the cell nucleus and in the mit...
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently...
The unwinding of nucleic acid secondary structures within cells is crucial to maintain genomic integ...
Guanine-rich nucleic acid sequences challenge the replication, transcription, and translation machin...
The concept that G-quadruplex (G4) structures can form within DNA or RNA in vitro has been long know...
Four-stranded nucleic acid structures called G-quadruplexes have been associated with important cell...
G-quadruplex (or G4) structures form in guanine-rich DNA sequences and threaten genome stability whe...
DFAD-box helicases are ubiquitous enzymes that catalyze the unwinding of energetically stable duplex...
Translation efficiency can be affected by mRNA stability and secondary structures, including G-quadr...
The identification of G-quadruplex (G4) binding proteins and insights into their mechanism of action...
G-quadruplexes (G4) are RNA and DNA secondary structures formed by the stacking of guanine quartets ...
International audienceG-rich sequences found at multiple sites throughout all genomes may form secon...
Members of the DEAD-box helicase family are involved in all fundamental processes of RNA metabolism,...
Eukaryotic translation initiation is a highly regulated process in protein synthesis. The principal ...
Helicases, enzymes that unwind DNA or RNA struc-ture, are present in the cell nucleus and in the mit...
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently...