Research Article Sponge-Like: A New Protocol for Preparing Bacterial Ghosts

License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications. In this study, for the first time we introduce a new protocol for BGs production. E. coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general protocol for BGs preparation.The protocol is based on using active chemical compounds in concentrations less than theMinimum Inhibition Concentration (MIC).Those chemical compounds are SDS, NaOH, andH 2