CHARACTERISTICS OF RAT MYOCARDIAL

TIn our investigation,we used short-time model of myocardial infarction of rats induced by high dose of isoproterenol (ISP). We investigated cardiac troponin T blood level (cTnT) and histologi-cal characteristics of rat myocardium. ISP, single, intraperitoneal dose mg/kg was given to male, adult, Wistar rats (n=). Rats were distributed depending on their body weight in subgroups: ISP I (BW -g) and ISP II (BW -g).Control group (n=) was treated with intraperitoneal dose of , NaCl. Cardiac TnT was measured by electrochemiluminiscence (ECLA) sandwich immunoassay in rat serum hours after ISP application. Rats ’ hearts were dissected and examined by qualitative histological method (HE). Statistical signifi cance was set at ,. Th ere was signifi cant diff erence in cTnT of ISP II (p=,) vs. control and ISP I (p<,) vs. control. Signifi cant diff erence was beetween ISP I and ISP II subgroups (p<.).Th e accent of histological changes of myocar-dium was on nuclei of cell. Cells showed acydophilic changes and nuclei disappearance as signs of coagulative necrosis development. Extensivity of histological changes were diff erent beetween ISP I and ISP II subgroup. Used dose of ISP induced development of myocardial necrosis in rats. Suben-docardial portion of myocardium was more vulnerability than subepicardial portion. Rats of ISP II had more extensive histological changes than these in ISP I. Administered doses of ISP enabled cTnT utilization as a marker of myocardial necrosis