Eff ect of interferon-γ on NF-κB and cytokine

Th e objective of our work was to evaluate the eff ect of interferon-γ (IFN-γ) on cytokine expression in rat acute pancreatitis (AP). AP was introduced to rats which were divided into Control, AP and IFN-γ group. Rats in the AP and IFN-γ group were sacrifi ced as , and h after IFN-γ treatment. Th e serum amylase (AMA), endotoxin and cytokines were detected. Th e pathological examination and immuno-fl uorescence staining of pancreas for TNF-α, NF-κB and IL- were performed. Th e serum AMA increased signifi cantly at h and reduced at h after AP. Th e increase in IFN-γ was higher than that in AMA. IL- in-creased in the AP and IFN group, and IFN increased markedly at h after AP. IL- reduced at h after AP compared with AP group. In the AP group, the immunostaining of cytokines increased. In the IFN group, the edema in the pancreas was more severe, and NF-κB and IL- expression was higher than that in the other two groups. IFN-γ can increase serum IL- and reduce IL- in AP. IFN-γ can increase serum IL- and reduce serum IL- in AP. Th e increase in NF-κB and IL- may exert infl uence on pro-infl ammatory cytokines to deteriorate infl ammation in the pancreas. Th us, to control the IFN-γ might has promise to attenuate pancreatitis