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Norovirus is a major cause of human gastroenteritis and has been identified as the predominant cause of food-borne outbreaks (1). Both food and water are important sources of norovirus trans-mission. Rapid and accurate detection is important for diagnosis of norovirus in patients and for tracing the source of the virus in food and environmental samples. Due to its high sensitivity and specificity, real-time PCR has become the gold standard for detection of norovirus from different sources (2, 3). Most real-time PCR assays for noroviruses target the conserved region in the junction betwee