Spatial buffering mechanism: mathematical model and computer simulations

(Communicated by Jianhong Wu) Abstract. It is generally accepted that the spatial buffering mechanism is im-portant to buffer extracellular-space potassium in the brain-cell microenviron-ment. In the past, this phenomenon, generally associated with glial cells, has been treated analytically and numerically using a simplified one-dimensional description. The present study extends the previous research by using a novel numerical scheme for the analysis of potassium buffering mechanisms in the extracellular brain-cell microenvironment. In particular, a lattice-cellular au-tomaton was employed to simulate a detailed two-compartment model of a two-dimensional brain-cell system. With this numerical approach, the present study elaborates upon previous theoretical work on spatial buffering (SB) by incorporating a more realistic structure of the brain-cell microenvironment, which was not feasible earlier. We use the experimental paradigm consisting of iontophoretic injection of KCl to study the SB mechanism. Our simulations confirmed the results reported in the literature obtained by an averaged model