For correspondence:Manivannan Rangasamy, Dept.of Pharmaceutics, JKKMMRF’s college of Pharmacy, B.

Acyclovir entrapped niosomes were prepared by hand shaking and ether injection process with different ratios of (1:1, 1;2 and 1:3) cholesterol (CHOL) and Span-80 (Non-ionic surfactant).The niosomes prepared were in the size range of 0.5-5 microns in the case of hand shaking process and 0.5-2.5 microns in the case of Ether injection process. The order of encapsulation efficiency increases when span-80 concentration was increased. In-vitro release study on acyclovir niosomes indicates 76.64 % release for formulation prepared with CHOL: Span-80 (1:1) and it takes an extended period of 1 day and 16 h for release