IJESMR

The study of micro propagation in Aloe vera and the investigation of developed a dependable protocol for large scale clonal multiplication of Aloe vera under the in vitro conditions. The Standardize optimum conditions for micropropagation of Aloe vera through a tissue culture. The Multiplication of shoots was found best on MS medium in combination of BA 2.0 mg/l, KN 0.5 mg/l and NAA 0.2 mg/l and the other good combinations of growth regulators in proliferating shoot were BA 2.0 mg/l and KN 0.2 mg/l (95.30%), followed by BA 2.0 mg/l (90.91%). MS medium containing BA and NAA was found to be the best medium in Aloe micropropagation Establishment of sterile axenic shoot culture from elite germplasm. Multiplication and elongation of micro shoots, Root formation and Hardening of multiplied shoots