Cell drug delivery of fluorescein loaded ApoB100 functionalized liposomes

Liposomes are often modified and functionalized with different ligands to control their biological properties, such as target intracellular penetration, in a desired fashion. Thus functionalized liposomes are taken into account for selectiv cells. The aim of this study is to check whether drug loaded ApoB100 functionalized liposomes have ability to be loaded with molecules, and to test their carrier properties for drugs by incubation with cultured cells. The vitro, where functionalized liposomes are internalized by clatrine coated endocytosis into malignant A375 cells. This drug deliver model involves the interaction of liposomes with cells via selective anchoring of ApoB100 ligand to the LDL receptor exposed membrane. The process is then followed by endocytosis of the liposome into the cell. This study was focused on preparation of fluorescein loaded liposomes, incubation with cultured cell and performing spectroscopic examination of cell lysates. Results showed that fluorescein loaded ApoB100-functionalized liposomes are recognized by LDL malignant cell via endocytosis at high rate. This justif delivering drugs to the target site, and further used as drug delivery systems for cancer therapy