Add to ORKGFluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a fluorophore depends on itsmolecular environment but not on its concentration. Molecular effects in a sample can therefore be investigated independently of the variable, and usually unknown concentration of the fluorophore. There is a variety of technical solutions of lifetime imaging in microscopy. The technical part of this paper focuses on time-domain FLIM by multidimensional time-correlated single photon counting, time-domain FLIM by gated image intensifiers, frequency-domain FLIM by gain-modulated image intensifiers, and frequency-domain FLIM by gain-modulated photomultipliers. The application part describes the most frequent FLIM applications: Measur...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environme...