Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco

Abstract Pre-harvest sprouting (PHS) of wheat is a major problem that severely limits the end-use quality of Xour in many wheat-growing areas worldwide. To identify quanti-tative trait loci (QTLs) for PHS resistance, a population of 171 recombinant inbred lines (RILs) was developed from the cross between PHS-resistant white wheat cultivar Rio Blanco and PHS-susceptible white wheat breeding line NW97S186. The population was evaluated for PHS in three greenhouse experiments and one Weld experiment. After 1,430 pairs of simple sequence repeat (SSR) primers were screened between the two parents and two bulks, 112 poly-morphic markers between two bulks were used to screen the RILs. One major QTL, QPhs.pseru-3AS, was identiWed in the distal region of chromosome 3AS and explained up to 41.0 % of the total phenotypic variation in three green-house experiments. One minor QTL, QPhs.pseru-2B.1, was detected in the 2005 and 2006 experiments and for the means over the greenhouse experiments, and explained 5.0–6.4 % of phenotypic variation. Another minor QTL, QPhs.pseru-2B.2, was detected in only one greenhouse experiment and explained 4.5 % of phenotypic variation for PHS resistance. In another RIL population developed from the cross of Rio Blanco/NW97S078, QPhs.pseru-3AS was signiWcant for all three greenhouse experiments and the means over all greenhouse experiments and explained up to 58.0 % of phenotypic variation. Because Rio Blanco is a popular parent used in many hard winter wheat breeding programs, SSR markers linked to the QTLs have potential for use in high-throughput marker-assisted selection of wheat cultivars with improved PHS resistance as well as Wne mapping and map-based cloning of the major QTL QPhs.pseru-3AS