Topics
high-performance liquid chromatographyTopical concept
amidaseBiomolecule
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mass spectrometryTopical concept
The peptide network of Streptococcue pneumoniae cell walls was solubilized using the pneumococcal au-tolytic amidase (N-acetylmuramoyl-L-alanine ami-dase, EC 3.5.1.28). The peptide material was fraction-ated into size classes by gel filtration followed by re-verse-phase high-performance liquid chromatography which resolved the peptide population into over 40 fractions. About 40 % of the lysines present participate in cross-links between stem peptides. The main com-ponents (3 monomers, 5 dimers, and 2 trimers), ac-counting for 77 % of all the wall peptides, were puri-fied. Their structures were determined using a combi-nation of amino acid and end-group analysis, mass spectrometry, and gas-phase sequencing. Two differ-ent types of cross-link...
Streptococcus pneumoniae is a Gram-positive bacterium capable of causing the life threatening invasi...
<p>(A) The PfbA architecture include the FSIRK and LPXTG sequence motifs that are common Gram-positi...
The peptidoglycan layer surrounding S. pneumoniae is essential for maintaining the shape of the cell...
The peptidoglycan of Gram-positive bacteria is known to trigger cytokine release from peripheral blo...
Understanding the molecular basis of bacterial cell wall assembly is of paramount importance in addr...
International audienceUnderstanding the molecular basis of bacterial cell wall assembly is of paramo...
<p>(A) Diagram of pneumococcal cell-wall composition, with LytA, LytB and lysozyme cleavage sites, a...
MurM is an aminoacyl ligase that adds L-serine or L-alanine as the first amino acid of a dipeptide b...
Evidence is given for the existence of at least four types of peptide cross-linkages in lysine-conta...
Peptidoglycan (PG) is an essential component of the bacterial cell envelope. This macromolecule cons...
A recombinant phage that produced a polypeptide possessing the characteristics of pneumolysin, the m...
<p>(A) SP, signal peptide; LysM, LysM peptidoglycan interaction domain. The extension of the fragmen...
The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in ...
<p>PgdA, an <i>N</i>-acetyl glucosamine, and Adr, an <i>O</i>-acetyltransferase, modify the MurNAc-G...
peer reviewedThe bacterial wall peptidoglycan is a network structure. Glycan strands of alternate 3,...
Streptococcus pneumoniae is a Gram-positive bacterium capable of causing the life threatening invasi...
<p>(A) The PfbA architecture include the FSIRK and LPXTG sequence motifs that are common Gram-positi...
The peptidoglycan layer surrounding S. pneumoniae is essential for maintaining the shape of the cell...
The peptidoglycan of Gram-positive bacteria is known to trigger cytokine release from peripheral blo...
Understanding the molecular basis of bacterial cell wall assembly is of paramount importance in addr...
International audienceUnderstanding the molecular basis of bacterial cell wall assembly is of paramo...
<p>(A) Diagram of pneumococcal cell-wall composition, with LytA, LytB and lysozyme cleavage sites, a...
MurM is an aminoacyl ligase that adds L-serine or L-alanine as the first amino acid of a dipeptide b...
Evidence is given for the existence of at least four types of peptide cross-linkages in lysine-conta...
Peptidoglycan (PG) is an essential component of the bacterial cell envelope. This macromolecule cons...
A recombinant phage that produced a polypeptide possessing the characteristics of pneumolysin, the m...
<p>(A) SP, signal peptide; LysM, LysM peptidoglycan interaction domain. The extension of the fragmen...
The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in ...
<p>PgdA, an <i>N</i>-acetyl glucosamine, and Adr, an <i>O</i>-acetyltransferase, modify the MurNAc-G...
peer reviewedThe bacterial wall peptidoglycan is a network structure. Glycan strands of alternate 3,...
Streptococcus pneumoniae is a Gram-positive bacterium capable of causing the life threatening invasi...
<p>(A) The PfbA architecture include the FSIRK and LPXTG sequence motifs that are common Gram-positi...
The peptidoglycan layer surrounding S. pneumoniae is essential for maintaining the shape of the cell...
