Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Elec-trophoresis 16:1090–1094, 1995). Important technical innovations in mass spectrometry (MS), protein identification methods, and database annotation, over the past decade, now make it possible to routinely identify thousands of proteins in complex biological samples (Nature 422:198–207, 2003). However, to gain new insights regarding fundamental biological questions, accurate protein quantification is also required. In this chapter, we present methods for the biochemical separation of different cellular com-partments, two-dimensional chromatographic separation of the constituent peptide populations, and the recently published Spectral Counting...
Protein quantification represents an important extension to identification proteomics, enabling the ...
Proteomics has enabled the direct investigation of biological material, at first through the analysi...
Nowadays identification and quantification of proteins from biological samples by mass spectrometry ...
Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Elec...
In recent years, mass spectrometry has moved more than ever before into the front line of protein-ce...
The increasing scale and complexity of quantitative proteomics studies complicate subsequent analysi...
Proteins are responsible for facilitating and regulating nearly all cellular processes, and the coll...
"Gel-free," or mass spectrometry (MS)-based, proteomics techniques are emerging as the methods of ch...
Recent innovations in liquid chromatography-mass spectrometry (LC-MS) based methods have facilitated...
Proteomics studies large-scale cellular functions directly at the protein level. In proteomics, mass...
With recent advances in experiment design, sample preparation, separation and instruments, mass spec...
In the current omics-age of research, major developments have been made in technologies that attempt...
Among a variety of global quantification strategies utilized in mass spectrometry (MS)-based proteom...
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ...
A major goal in proteomics is the comprehensive and accurate description of a proteome. This task in...
Protein quantification represents an important extension to identification proteomics, enabling the ...
Proteomics has enabled the direct investigation of biological material, at first through the analysi...
Nowadays identification and quantification of proteins from biological samples by mass spectrometry ...
Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Elec...
In recent years, mass spectrometry has moved more than ever before into the front line of protein-ce...
The increasing scale and complexity of quantitative proteomics studies complicate subsequent analysi...
Proteins are responsible for facilitating and regulating nearly all cellular processes, and the coll...
"Gel-free," or mass spectrometry (MS)-based, proteomics techniques are emerging as the methods of ch...
Recent innovations in liquid chromatography-mass spectrometry (LC-MS) based methods have facilitated...
Proteomics studies large-scale cellular functions directly at the protein level. In proteomics, mass...
With recent advances in experiment design, sample preparation, separation and instruments, mass spec...
In the current omics-age of research, major developments have been made in technologies that attempt...
Among a variety of global quantification strategies utilized in mass spectrometry (MS)-based proteom...
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the ...
A major goal in proteomics is the comprehensive and accurate description of a proteome. This task in...
Protein quantification represents an important extension to identification proteomics, enabling the ...
Proteomics has enabled the direct investigation of biological material, at first through the analysi...
Nowadays identification and quantification of proteins from biological samples by mass spectrometry ...