Figure 6. The effect of siRNA-mediated downregulation of

members of the HH cascade on proliferation of NT2 cells. Proliferation was assessed by the MTT assay 5 days after siRNA transfection and the figure represents the mean of three independent experiments +/- SE. * p < 0,01. Figure 3. Proliferation of NT2 cells in response to KAAD cyclopamine treatment compared to the inactive Cyclopamine analogue Tomatidine seen over 5 days. Data is presented as percent of proliferation in mock-treated cells and represents the results obtained in three independent experiments. Bars: +/- SE. * p < 0,01. siRNA mediated knock-down of the Hedgehog pathway member GLI1 inhibits the proliferation of NT2 neuronal precursor cell